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Bionic micro-nano interface and circulating tumor cells in vitro culture is a use of bionic characteristics of micro-nano structure as a surface enhanced Raman spectroscopy (SERS) base, with circulating tumor cells (CTCs) specific binding, and then through in vitro culture method, high sensitivity and selectivity of CTCs detection and analysis of a new technology. Biomimetic micro-nano interface is a micro-nano structure mimicking the structure and function of biological surface, which is highly biocompatible, programmable and controllable, and can realize various functions and interactions, such as drug delivery, biosensing, immune regulation, etc. This technique can exploit the high selectivity, high efficiency and high throughput of bionic micro-nano interfaces, enabling the detection and analysis of CTCs.

A fluid multivalent biomimetic micro-nano interface based on aptamer functionalized leukocyte membrane nanovicles (WNVs) for efficient capture and in vitro culture of CTCs. The interface uses WNVs as SERS active nanostructures, functionalize them with the aptamer (aptamer), and specifically binds them to tumor markers (such as epidermal growth factor receptor 2, HER 2) present on the surface of CTCs. Then, by Raman spectroscopy, the Raman signals of aptamers and WNVs are detected to enable quantitative and qualitative analysis of CTCs. Meanwhile, the interface can also transfer captured CTCs into culture plates and culture them in vitro under appropriate culture conditions, thus enabling further phenotypic and functional analysis of CTCs.

A biomimetic micro-nano interface based on polyglycol-polypropylene (PEG-PBA) copolymer and gold nanorods (AuNRs) for the isolation and analysis of CTCs. This interface uses PEG-PBA copolymers as a SERS active nanostructure, composite them with AuNRs and targeted binding to tumor markers (e. g. prostate specific antigen, PSA) present on the surface of CTCs. Then, the Raman signals of PEG-PBA copolymers and AuNRs were detected by Raman spectroscopy, thus enabling a quantitative and qualitative analysis of CTCs. Meanwhile, this interface can also transfer the isolated CTCs to culture plates and culture them in vitro under appropriate culture conditions, thus enabling further phenotypic and functional analysis of CTCs.

A biomimetic nanofiber interface based on polygylated-polycaprolactone (PEG-PCL) copolymers and ferrite (Fe3O4) nanoparticles for in vitro culture and analysis of CTCs. The interface utilizes the PEG-PCL copolymer as the SERS active nanostructure, recombining it with Fe3O4 nanoparticles and preparing it into nanofiber membranes by electrospinning. This membrane can be used as an in vitro culture substrate for CTCs to provide a three-dimensional biomimetic microenvironment to promote the proliferation and differentiation of CTCs. At the same time, the membrane can also detect the Raman signal of PEG-PCL copolymer and Fe3O4 nanoparticles through the method of Raman spectroscopy, thus realizing the quantitative and qualitative analysis of CTCs.

Reference Documentation: 

[1]Fluidic multivalent bionanointerfaces for efficient capture and culture of circulating tumor cells.

[2]Biomimetic nanointerfaces for circulating tumor cell isolation and analysis.